Coding

Part:BBa_K5252005:Design

Designed by: Samuel Williams   Group: iGEM24_EPFL   (2024-09-28)


XplA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 178
    Illegal BglII site found at 963
    Illegal BamHI site found at 85
    Illegal BamHI site found at 1398
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 178
    Illegal NgoMIV site found at 288
    Illegal NgoMIV site found at 937
    Illegal NgoMIV site found at 1557
    Illegal AgeI site found at 343
    Illegal AgeI site found at 460
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Codon optimized for E. coli while removing SapI, BsaI, BbsI, PaqCI, Esp3I, FaqI restriction sites using Genscript.

- Addition of 3xFLAG-tag and GS-linker sequence



Source

From Rhodococcus rhodochrous

References